Jul 5, 2016




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Coagulation

Lupus Anticoagulant Work-Up

Lupus Anticoagulant Work-Up (HCLUPUS) Coagulation Lab Test

Name: Lupus Anticoagulant Work-Up

Test Name: Lupus Anticoagulant Work-Up

Health Link Test Code: HCLUPUS

LIS Test Code: LUPUS

CPT Code(s): 85730, 85732, 85613; Additional CPT codes may be added for testing-85610, 85730x2, 85732x2, 85670, 85240, 85597.

Test Component:

Includes APTT, Clot Time Silica and dilute Russell Viper Venom Screen.  May reflex to Protime, APTT Inhibitor Screen, Thrombin Time, Factor VIII Assay and dilute Russell Viper Venom Confirm.

Clinical Information:

May be part of hypercoagulation workup. May also be performed as a result of an elevated APTT.

Days Performed: Mon and Thurs, dayshift- specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call 263-5005 before collection.

In-Lab Turnaround Time: 4 - 10 days.

Stat In-Lab Turnaround Time: 1 day.

Patient Preparation:

Patient must not have received any of the following within 24 hours of collection: unfractionated heparin, low molecular weight heparin, fondaparinux, bivalirudin, dabigatran, rivaroxiban or apixaban.

Specimen: Blood

Collection Container: Light blue top (3.2% NaCitrate)

Collection Instructions:

Do NOT collect through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelets. Tube must be allowed to fill completely.


Indicate if patient is receiving anticoagulant (warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy and duration.

Collection Volume: Four full 3.5 mL tubes

Pediatric Collection Volume: 2 full pediatric tubes filled to mark

Stability Ambient:

4 hours on spun tube

Stability Refridgerated:

Not acceptable

Stability Frozen:

2 weeks at -20°C; 6 months at -70°C

Sample Analyzed: Plasma

Testing Volume: 5 mL

Pediatric Testing Volume: 1.5 mL

Specimen Processing:

Ultracentrifuge sample and label "ULT". If an ultracentrifuge is not available, centrifuge specimen to yield platelet poor plasma (platelet count should be 10 K/µL or less). Separate plasma and transfer to plastic tube and spin again. Transfer plasma from second spin to clean plastic tube or vial. Indicate on tube "spun twice". Freeze plasma within 4 hours of collection at -20°C or below.

Specimen Transport:

Transport whole blood specimen at room temperature to laboratory within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen plasma aliquot on dry ice to UWHC Hospital Laboratory.

Unacceptable Criteria:

Specimens collected from patients who have received heparin within 72 hours of collection, a direct thrombin inhibitor within 24 hours of collection or rivaroxaban within 24 hours of collection are not acceptable. Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.



Interpretation Type: Expected Results

Additional Information:

The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.