Name: ADAMTS13 Activity, Reflex to Inhibitor
Test Name: ADAMTS13 Activity, Reflex to Inhibitor
Health Link Test Code: ADAMACT
LIS Test Code: ADAMTS13
CPT Code(s): 85397
Test is used to assist in diagnosing congenital or inherited thrombotic thrombocytopenic purpura (TTP).
Days Performed: Daily- Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call (608)263-5005 before collection.
In-Lab Turnaround Time: 2 days.
Stat In-Lab Turnaround Time: 1 day.
Collection Container: Light blue top (3.2% NaCitrate)
Collection Volume: Full 3.5 mL tube
Pediatric Collection Volume: Pediatric tube filled to fill mark
2 hours unspun
2 months at -200 C
Testing Volume: 0.5 mL
Pediatric Testing Volume: 0.3 mL
Centrifuge specimen to yield platelet poor plasma (platelet count should be less than 10K/µL). Separate plasma and transfer to a plastic tube or vial. Freeze plasma at -200C.
Transport whole blood specimen at room temperature to UWHC Hospital Laboratory to be received within 2 hours of collection. If unable to transport the specimen to reach the UWHC lab within 2 hours see Specimen Processing for instructions. Transport frozen aliquot on dry ice to UWHC Hospital Laboratory.
Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater then 2 hours old when received by the laboratory are not acceptable.
Results > 60% are considered normal. Results < 10% ADAMTS13 are highly indicative of thrombotic thrombocytopenia purpura (TTP) in an appropriate clinical setting.
If the ADAMTS13 Activity is < 10% an ADAMTS13 inhibitor order will reflex and the sample will be sent to a reference laboratory for inhibitor testing.
The performance charateristics of this test were validated by the UWHC Clinical Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test, however, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management.