Name: PSA Total, Screen
Test Name: PSA Total, Screen
Health Link Test Code: PSAM
LIS Test Code: PSAS
CPT Code(s): G0103
Methodology: Chemiluminescent Immunoassay
Days Performed: Daily, 24 hours.
In-Lab Turnaround Time: 4 hours.
Stat In-Lab Turnaround Time: 1 hour.
Collection Container: Red cap with yellow ring (SST)
Also Acceptable: Red top
Collection Volume: 2 mL
Pediatric Collection Volume: 0.3 mL
Sample Analyzed: Serum
Testing Volume: 1 mL
Pediatric Testing Volume: 0.15 mL
Centrifuge. If a complete barrier has not formed, transfer cell-free serum to plastic vial. Refrigerate.
Transport entire specimen to the laboratory. Transport with coolant pack if coming from clinic location. Send frozen if sample will be more than 24 hours old when it arrives.
18 years and up: 0.00-4.00 ng/mL
Interpretation Type: Reference Interval
Testing performed on Abbott Architect i2000.
Samples should be obtained before biopsy, prostatectomy or prostatic massage, since manipulation of the prostate gland may lead to elevated PSA levels persisting for up to 3 weeks.
Studies have shown conflicting results on the existence of an effect of digital rectal examination on PSA level. Therefore, when possible, obtain PSA samples before digital rectal examination.
PSA expression may be altered due to hormonal therapy for prostate cancer. Consequently, a low PSA result following a prostatic cancer treatment which includes hormonal therapy may not adequately reflect the presence of residual or recurrent disease.
Results may be affected if patient has developed Human Anti-Murine Antibodies (HAMA) due to exposure to murine based products.
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with in vitro immunoassays. Samples from patients routinely exposed to animals or animal serum products can demonstrate this type of interference potentially causing an anomalous result.
A high dose hook effect is not seen up to 48,000 ng/mL.
The PSA assay may be used in conjunction with a digital rectal exam as a screening test for prostate cancer.