Nov 20, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Chemistry

Hemoglobin A1C

Hemoglobin A1C (HA1C) Chemistry Lab Test

Name: Hemoglobin A1C

Test Name: Hemoglobin A1C

Health Link Test Code: HA1C

LIS Test Code: A1C

CPT Code(s): 83036

Test Component:

A1C is measured and reported along with the estimated Average Glucose (eAG).

Methodology: Ion Exchange Chromatography/Spectrometry

Clinical Information:

Hemoglobin A1C is the major subfraction of glycated hemoglobin. Glycated hemoglobin is the combination of glucose with hemoglobin in a nonenzymatic reaction, which is continuous during the lifespan of the erythrocyte. The concentration of HbA1C provides an index of the adequacy of long term blood glucose control in patients with diabetes. The HbA1C level indicates the glucose control over the previous 8 to 10 weeks, whereas blood glucose reflects the metabolic control for only the previous 24 hours. Intravenous glucose therapy does not typically affect the HbA1C level.


Conditions that affect erythrocyte turnover (hemolysis, blood loss) and hemoglobin variants must be considered, particularly when the A1c result does not correlate with the patient's clinical presentation.

Days Performed: Daily.

In-Lab Turnaround Time: 1 day.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Lavender top

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

24 hours

Stability Refridgerated:

7 days

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.1 mL

Specimen Processing:

Refrigerate specimen.

Specimen Transport:

Transport specimen to Laboratory. Transport with coolant pack if coming from clinic location.



Reference interval:   

         4.0-5.6 %

  6 years and up
Interpretive guideline:  
   Level of control

             < 7.1 %


          7.1-8.0 %


          8.1-9.0 %


             > 9.0 %




Effective Date of Reference Range: Mon, 17 Jul 2017 13:30:00 CDT

Test Limitations:

Hemoglobin A1C requests more frequent than 90 days will automatically be cancelled by the laboratory as a duplicate request.


Patient specimens displaying a hemoglobin variant known to interfere with this assay will be sent to a reference lab where the  A1C method is less affected by most hemoglobin variants.

Patient specimens with an A1c value > 6.5% displaying any hemoglobin variant will be sent to a reference lab where the  A1C method is less affected by most hemoglobin variants.

Additional Information:

The NGSP has certified this method and its reagents as having documented traceability to the Diabetes Control and Complications Trial Reference Method.


An estimated Average Glucose (eAG) is provided with the A1C result.