Jan 8, 2018




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Chemistry


Alpha-Fetoprotein (XAFPTM) Chemistry Lab Test

Name: Alpha-Fetoprotein

Test Name: Alpha-Fetoprotein

Health Link Test Code: XAFPTM

LIS Test Code: AFP

CPT Code(s): 82105

Methodology: Chemiluminescent Immunoassay

Days Performed: Daily, 24 hours.

In-Lab Turnaround Time: 4 hours.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red cap with yellow ring (SST)

Also Acceptable: Red top

Collection Volume: 1 mL

Pediatric Collection Volume: 0.5 mL

Stability Ambient:

3 days

Stability Refridgerated:

7 days

Stability Frozen:

>7 days

Sample Analyzed: Serum

Testing Volume: 0.5 mL

Pediatric Testing Volume: 0.15 mL

Specimen Processing:

Centrifuge. If a complete barrier has not formed, transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.


8 months and up:  0.0-8.8 ng/mL


Reference range for newborns is not available but concentrations over 200,000 have been reported in newborns.  AFP gradually declines as the infant matures.  At 8 months of age, the normal AFP level of infants approaches the adult level. 

Pediatric Reference:  Serum Alpha Fetoprotein (AFP) Levels in Normal Infants, Pediatr. Res 15:50-52 1981

Interpretation Type: Expected Results

Effective Date of Reference Range: Mon, 10 Aug 2015 00:00:00 CDT

Test Limitations:

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.

A high dose hook effect is not seen up to 10,000,000 ng/mL.