MicroClave® Clear – Drawing Blood through Your Child’s Central
Line Using a Syringe
Using a central line to draw blood is a safe, easy way to draw blood samples needed to diagnose
and treat your child. This handout explains how you can safely do this at home. For young
infants, flushing volumes may vary. Please talk with your doctor, nurse, or nurse practitioner.
Angel Wing Transfer Device
2-3 5 mL syringes
1-2 10 mL syringe(s) of saline or ______ mL, as specified
Blood tubes for samples (color depends on orders)
1 5mL syringe of heparinized saline or _______ mL, as specified
Steps: Before going home, a nurse will show you how you can safely do these steps at home.
1. Wash your hands and put on gloves.
2. Clean the top of the needleless connector (MicroClave® Clear) with an alcohol wipe for 15
seconds and allow to dry.
3. Using sterile technique, with central line clamped, attach an empty 5 mL syringe to the
4. Unclamp catheter and pull back slowly on the syringe to draw 3 mL of “waste” blood into
the syringe if the line was capped, 5ml if the line was running. This will remove the
heparinized saline that is in the line.
5. Remove the “waste” syringe and attach a new 5 mL syringe (or the size of syringe needed to
draw the volume of blood required for the ordered labs).
6. Pull back slowly on the syringe to draw the amount of blood needed to fill the blood tube (s).
If blood does not flow into the tube: Flush the line through the needleless connector
(MicroClave® Clear) with 10 mL of saline using a push-stop-push method. Then, repeat
steps 1 through 5. If there is still no blood flow, contact your child’s doctor or nurse.
7. Repeat step 5 if more than 5 mL of blood is needed to fill the blood tubes.
8. When finished, remove the syringe.
9. Flush the line through the needleless connector (MicroClave® Clear) with 10 mL (or _____
mL) of normal saline using a push stop push method followed by 5 mL (or________mL)
of heparinized saline.
After flushing, if you can see blood in the needleless connector, remove the connector and
replace with a new one. See “Changing the Needleless Connector” (MicroClave® Clear) below.
10. Clamp the line while holding down the plunger and remove syringe at the same time.
11. Remove cap from angel wing transfer device. Attach the syringe filled with blood.
12. Push the blood tube onto the inner needle of the angel wing transfer device. Blood should
enter the tube. Blood will not fully fill the tube. This is normal. Do not try to push more
blood into the tube.
13. When blood flow stops, attach next blood tube. If more than 5 mL of blood is needed,
always attach the syringe of blood to the angel wing before you attach the blood tube.
14. When finished, put the angel wing device and all the syringes into a plastic needle disposal
Changing the needleless connector (MicroClave® Clear)
The needleless connector needs to be changed every three days or as directed
by your homecare provider. Note: change sooner if blood is visible in the
connector. The connector is sterile at the connector end.
1. Wash your hands for 60 seconds.
2. Remove the plastic cover from the syringe. Push air out of the syringe.
Open a 4x4 sterile gauze and drop it onto the sterile field.
3. Put on mask and sterile gloves.
4. Open the needleless connector package. Use one piece of sterile gauze to pick up your saline
syringe. Connect the syringe to the end of the connector. Twist on completely. If the tip of
the syringe touches anything other than the end of the catheter, stop, and replace the syringe.
5. Fill the needleless connector with saline solution by pointing the blue protective cap found at
the other end of the connector up towards the ceiling. This will remove all air from the
connector. Leave the syringe filled with saline attached to the connector.
6. Open one of your alcohol pads. Hold asterile 4x4 gauze in your non-dominant hand and pick
up your child’s line above the old connector.
7. Cleanse the connection between the connector and your child’s line with an alcohol wipe
for 15-20 seconds and let it dry
8. Clamp the catheter line.
9. Remove the old needleless connector from the catheter. Hold the line without touching the
end of the catheter opening.
10. Remove the blue protective cap from the new needleless connector. Attach the connector
to the end of the catheter. Do not over tighten. One firm turn onto the line is sufficient.
11. Unclamp the line.
12. Flush the line using push- stop- push method with the remaining flush solution already
attached to the needleless connector.
13. As you complete the flush, clamp the line while holding down the plunger and removing
syringe at the same time.
Your health care team may have given you this information as part of your care. If so, please use it and call if you
have any questions. If this information was not given to you as part of your care, please check with your doctor.
This is not medical advice. This is not to be used for diagnosis or treatment of any medical condition. Because
each person’s health needs are different, you should talk with your doctor or others on your health care team
when using this information. If you have an emergency, please call 911. Copyright © 7/2016 University of
Wisconsin Hospitals and Clinics Authority. All rights reserved. Produced by the Department of Nursing. HF#5888