Midstream Urine Sample -- Female
Before you start, be sure to read all the instructions. Please ask questions if you are unsure
about any of these steps. Follow these steps carefully.
1. Lower the toilet seat, if needed.
2. Wash your hands with soap and water. Dry your hands with clean paper towels.
3. Open the urine container. Do not touch the inside of the container or cover. Open the
4. Clean the urinary area.
ξ Find a stable position, either sitting on or squatting above the toilet.
ξ Use the forefingers of one hand to separate the folds (labia) around your urinary
opening. Keep the labia apart while you clean.
ξ Clean the right side of the labia with one towelette. Wipe slowly from front to back
once. Throw away the towelette. With a second towelette, clean the left side the
same way. Toss the towelette in a nearby wastebasket.
ξ Clean the center area with the third towelette. Wipe slowly from front to back once.
Then throw away the towelette.
5. Keep the labia apart, and urinate a small amount into the toilet. While urinating, move the
container into the urine stream to catch a sample. Stop the flow and finish urinating into the
toilet. The container does not have to be filled to the top.
6. Screw the cover onto the urine container. Dry the outside of container if it is wet. Wash your
7. Give the sample to the clinic staff.
If you make a mistake in collecting the specimen, please ask for a new container and
The Spanish version of this Health Facts for You is #5479.
Your health care team may have given you this information as part of your care. If so, please use it and call if you
have any questions. If this information was not given to you as part of your care, please check with your doctor. This
is not medical advice. This is not to be used for diagnosis or treatment of any medical condition. Because each
person’s health needs are different, you should talk with your doctor or others on your health care team when using
this information. If you have an emergency, please call 911. Copyright © 6/2016 University of Wisconsin Hospitals
and Clinics Authority. All rights reserved. Produced by the Department of Nursing HF#4236